SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06257

Signal sequence   MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLEGDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKR

Length  85

Organism  Saccharomyces cerevisiae

UniProt ID  NA,

Motif  1..85



Activity Information


Pathway  SRP-dependent

Natural/Artificial  Artificial

Subcellular location  ER

Secretion efficiency  NA

Type  Sec/SPI



Structure Information


PDB ID  6KRL,

Predicted Structure ID  SPKB06257.cif

Structure  SPKB06257.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  8939.01224

Aliphatic index  95.41176471

Isoelectric point  3.84316662

Charge (pH=7)   -6.989251009

Topological polar surface area  3661.15

Aromaticity  0.082352941

Average rotatable bonds  3.388235294

Number of hydrogen bond donors  124

Number of hydrogen bond acceptors  125

X logP energy  -40.01226

Hydropathy 



Literature Information


Title  Comparison of Different Signal Peptides for the Efficient Secretion of the Sweet-Tasting Plant Protein Brazzein in Pichia pastoris

DOI  10.3390/life11010046,

Publication Year  2021

PubMed ID  33450886,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.