SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06193

Signal sequence   MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAATDA

Length  46

Organism  Escherichia coli

UniProt ID  NA,

Motif  1..46



Activity Information


Pathway  Tat

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  NA

Type  Tat/SPI



Structure Information


PDB ID  9H4T,

Predicted Structure ID  SPKB06193.cif

Structure  SPKB06193.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  4844.54724

Aliphatic index  85.2173913

Isoelectric point  12.32397707

Charge (pH=7)   2.998860711

Topological polar surface area  2119.26

Aromaticity  0.043478261

Average rotatable bonds  3.456521739

Number of hydrogen bond donors  74

Number of hydrogen bond acceptors  68

X logP energy  -26.90205

Hydropathy 



Literature Information


Title  N-Terminal Fused Signal Peptide Prompted Extracellular Production of a Bacillus-Derived Alkaline and Thermo Stable Xylanase in E. coli Through Cell Autolysis

DOI  10.1007/s12010-020-03323-9,

Publication Year  2020

PubMed ID  32382941,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.