SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06122

Signal sequence   MNKKVLTLSAVMASMLFGAAAHA

Length  23

Organism  Escherichia coli

UniProt ID  NA,

Motif  1..23



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  NA

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB06122.cif

Structure  SPKB06122.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2362.89234

Aliphatic index  102.173913

Isoelectric point  10.80542203

Charge (pH=7)   2.088303323

Topological polar surface area  888.02

Aromaticity  0.043478261

Average rotatable bonds  3.434782609

Number of hydrogen bond donors  31

Number of hydrogen bond acceptors  34

X logP energy  -7.7684

Hydropathy 



Literature Information


Title  In silico analysis of different signal peptides for the secretory production of recombinant human keratinocyte growth factor in Escherichia coli

DOI  10.1016/j.compbiolchem.2019.03.003,

Publication Year  2019

PubMed ID  30999249,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.