SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB06014

Signal sequence   MKKIWLALAGLVLAFSASA

Length  19

Organism  Escherichia coli

UniProt ID  NA,

Motif  1..19



Activity Information


Pathway  Sec

Natural/Artificial  Artificial

Subcellular location  Periplasmic space

Secretion efficiency  NA

Type  Sec/SPI



Structure Information


PDB ID  8XFP,

Predicted Structure ID  SPKB06014.cif

Structure  SPKB06014.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  1990.47774

Aliphatic index  144.2105263

Isoelectric point  10.80537956

Charge (pH=7)   1.997394232

Topological polar surface area  695.41

Aromaticity  0.105263158

Average rotatable bonds  3.421052632

Number of hydrogen bond donors  25

Number of hydrogen bond acceptors  25

X logP energy  -2.2955

Hydropathy 



Literature Information


Title  Novel signal peptides improve the secretion of recombinant Staphylococcus aureus Alpha toxin(H35L) in Escherichia coli

DOI  10.1186/s13568-017-0394-1,

Publication Year  2017

PubMed ID  28497288,

Patent ID  NA,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.