SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB00704

Signal sequence   MHYKKAFLASLLSSIALTAYAPPEPWATLTPSSKMDGGTTEYRTSFGLAVIPFTVTESKVKR

Length  62

Organism  Artificial

UniProt ID  NA,

Motif  1..62



Activity Information


Pathway  SRP-dependent

Natural/Artificial  Artificial

Subcellular location  ER

Secretion efficiency  NA

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB00704.cif

Structure  SPKB00704.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  6765.84514

Aliphatic index  75.64516129

Isoelectric point  10.00636931

Charge (pH=7)   3.090632208

Topological polar surface area  2606.18

Aromaticity  0.112903226

Average rotatable bonds  3.435483871

Number of hydrogen bond donors  93

Number of hydrogen bond acceptors  95

X logP energy  -24.12546

Hydropathy 



Literature Information


Title  Pichia pastoris PIR1 secretion signal peptide for recombinant protein expression and Pichia pastoris PIR1 and PIR2 anchor domain peptides for recombinant surface display

DOI  NA,

Publication Year  2010

PubMed ID  NA,

Patent ID  US7741075B2,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.