SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB00250

Signal sequence   MALLHKNMKHLWFFLLLVAAPRWVLS

Length  26

Organism  Artificial

UniProt ID  NA,

Motif  1..26



Activity Information


Pathway  SRP-dependent

Natural/Artificial  Artificial

Subcellular location  ER

Secretion efficiency  Nivolumab titer: 6.1g/L

Type  Sec/SPI



Structure Information


PDB ID  7MX8,

Predicted Structure ID  SPKB00250.cif

Structure  SPKB00250.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  3135.91404

Aliphatic index  138.8461538

Isoelectric point  11.82306892

Charge (pH=7)   3.179209102

Topological polar surface area  1048.23

Aromaticity  0.153846154

Average rotatable bonds  3.807692308

Number of hydrogen bond donors  37

Number of hydrogen bond acceptors  36

X logP energy  0.47907

Hydropathy 



Literature Information


Title  Novel combination of TIS sequences and signal peptide sequences for expression of recombinant proteins

DOI  NA,

Publication Year  2022

PubMed ID  NA,

Patent ID  CN115298313A,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.