SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB00179

Signal sequence   MFRKSLCMLIGSCLLSSVLA

Length  20

Organism  Artificial

UniProt ID  NA,

Motif  1..20



Activity Information


Pathway  SRP-dependent

Natural/Artificial  Artificial

Subcellular location  ER

Secretion efficiency  Increase thermoresistant mannanase expression by 1.85 times

Type  Sec/SPI



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB00179.cif

Structure  SPKB00179.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2172.74874

Aliphatic index  136.5

Isoelectric point  8.828181491

Charge (pH=7)   1.873736522

Topological polar surface area  785.06

Aromaticity  0.05

Average rotatable bonds  3.7

Number of hydrogen bond donors  31

Number of hydrogen bond acceptors  31

X logP energy  -5.83663

Hydropathy 



Literature Information


Title  Modified signal peptide for guiding secretory expression of heat-resistant mannase, recombinant plasmid, genetically engineered bacterium, production method and application

DOI  NA,

Publication Year  2020

PubMed ID  NA,

Patent ID  CN111471087A,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.