SPKB

Signal Peptide Knowledge Base

General Information


SPKB ID  SPKB00174

Signal sequence   MKFSFISAAVAASVIWGASAGPLSEAHA

Length  28

Organism  Artificial

UniProt ID  NA,

Motif  1..28



Activity Information


Pathway  NA

Natural/Artificial  Artificial

Subcellular location  Extracellular environment

Secretion efficiency  Alkaline protease activity: 2217U/mL

Type  NA



Structure Information


PDB ID  NA,

Predicted Structure ID  SPKB00174.cif

Structure  SPKB00174.cif
Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)
Loading PAE…
Expected Position Error (Ångströms)


Physicochemical Information


Molecular weight  2777.19024

Aliphatic index  91.07142857

Isoelectric point  7.549726882

Charge (pH=7)   0.090373479

Topological polar surface area  1049.17

Aromaticity  0.107142857

Average rotatable bonds  3.035714286

Number of hydrogen bond donors  37

Number of hydrogen bond acceptors  38

X logP energy  -10.25

Hydropathy 



Literature Information


Title  Signal peptide mutant capable of enhancing expression levels of exogenous alkaline protease, and construction method and applications thereof

DOI  NA,

Publication Year  2020

PubMed ID  NA,

Patent ID  CN111153968A,



Faculty of Applied Sciences, Macao Polytechnic University, Macao, China.