# By using this file you agree to the legally binding terms of use found at # https://github.com/google-deepmind/alphafold3/blob/main/OUTPUT_TERMS_OF_USE.md. # To request access to the AlphaFold 3 model parameters, follow the process set # out at https://github.com/google-deepmind/alphafold3. You may only use these if # received directly from Google. Use is subject to terms of use available at # https://github.com/google-deepmind/alphafold3/blob/main/WEIGHTS_TERMS_OF_USE.md. data_spkb29472 # _entry.id spkb29472 # loop_ _atom_type.symbol C N O S # loop_ _audit_author.name _audit_author.pdbx_ordinal "Google DeepMind" 1 "Isomorphic Labs" 2 # _audit_conform.dict_location https://raw.githubusercontent.com/ihmwg/ModelCIF/master/dist/mmcif_ma.dic _audit_conform.dict_name mmcif_ma.dic _audit_conform.dict_version 1.4.5 # loop_ _chem_comp.formula _chem_comp.formula_weight _chem_comp.id _chem_comp.mon_nstd_flag _chem_comp.name _chem_comp.pdbx_smiles _chem_comp.pdbx_synonyms _chem_comp.type "C6 H15 N4 O2" 175.209 ARG y ARGININE C(C[C@@H](C(=O)O)N)CNC(=[NH2+])N ? "L-PEPTIDE LINKING" "C3 H7 N O2 S" 121.158 CYS y CYSTEINE C([C@@H](C(=O)O)N)S ? "L-PEPTIDE LINKING" "C6 H13 N O2" 131.173 LEU y LEUCINE CC(C)C[C@@H](C(=O)O)N ? "L-PEPTIDE LINKING" "C6 H15 N2 O2" 147.195 LYS y LYSINE C(CC[NH3+])C[C@@H](C(=O)O)N ? "L-PEPTIDE LINKING" "C5 H11 N O2 S" 149.211 MET y METHIONINE CSCC[C@@H](C(=O)O)N ? "L-PEPTIDE LINKING" "C9 H11 N O2" 165.189 PHE y PHENYLALANINE c1ccc(cc1)C[C@@H](C(=O)O)N ? "L-PEPTIDE LINKING" "C3 H7 N O3" 105.093 SER y SERINE C([C@@H](C(=O)O)N)O ? "L-PEPTIDE LINKING" "C5 H11 N O2" 117.146 VAL y VALINE CC(C)[C@@H](C(=O)O)N ? "L-PEPTIDE LINKING" # _citation.book_publisher ? _citation.country UK _citation.id primary _citation.journal_full Nature _citation.journal_id_ASTM NATUAS _citation.journal_id_CSD 0006 _citation.journal_id_ISSN 0028-0836 _citation.journal_volume 630 _citation.page_first 493 _citation.page_last 500 _citation.pdbx_database_id_DOI 10.1038/s41586-024-07487-w _citation.pdbx_database_id_PubMed 38718835 _citation.title "Accurate structure prediction of biomolecular interactions with AlphaFold 3" _citation.year 2024 # loop_ _citation_author.citation_id _citation_author.name _citation_author.ordinal primary "Google DeepMind" 1 primary "Isomorphic Labs" 2 # _entity.id 1 _entity.pdbx_description . _entity.type polymer # _entity_poly.entity_id 1 _entity_poly.pdbx_strand_id A _entity_poly.type polypeptide(L) # loop_ _entity_poly_seq.entity_id _entity_poly_seq.hetero _entity_poly_seq.mon_id _entity_poly_seq.num 1 n MET 1 1 n LEU 2 1 n ARG 3 1 n LEU 4 1 n CYS 5 1 n PHE 6 1 n LEU 7 1 n LEU 8 1 n SER 9 1 n PHE 10 1 n MET 11 1 n CYS 12 1 n LEU 13 1 n VAL 14 1 n LYS 15 1 n SER 16 # _ma_data.content_type "model coordinates" _ma_data.id 1 _ma_data.name Model # _ma_model_list.data_id 1 _ma_model_list.model_group_id 1 _ma_model_list.model_group_name "AlphaFold-beta-20231127 (3.0.0 @ 2025-07-03 21:22:40)" _ma_model_list.model_id 1 _ma_model_list.model_name "Top ranked model" _ma_model_list.model_type "Ab initio model" _ma_model_list.ordinal_id 1 # loop_ _ma_protocol_step.method_type _ma_protocol_step.ordinal_id _ma_protocol_step.protocol_id _ma_protocol_step.step_id "coevolution MSA" 1 1 1 "template search" 2 1 2 modeling 3 1 3 # loop_ _ma_qa_metric.id _ma_qa_metric.mode _ma_qa_metric.name _ma_qa_metric.software_group_id _ma_qa_metric.type 1 global pLDDT 1 pLDDT 2 local pLDDT 1 pLDDT # _ma_qa_metric_global.metric_id 1 _ma_qa_metric_global.metric_value 73.48 _ma_qa_metric_global.model_id 1 _ma_qa_metric_global.ordinal_id 1 # loop_ _ma_qa_metric_local.label_asym_id _ma_qa_metric_local.label_comp_id _ma_qa_metric_local.label_seq_id _ma_qa_metric_local.metric_id _ma_qa_metric_local.metric_value _ma_qa_metric_local.model_id _ma_qa_metric_local.ordinal_id A MET 1 2 66.21 1 1 A LEU 2 2 69.21 1 2 A ARG 3 2 67.61 1 3 A LEU 4 2 78.28 1 4 A CYS 5 2 79.15 1 5 A PHE 6 2 73.74 1 6 A LEU 7 2 79.19 1 7 A LEU 8 2 78.46 1 8 A SER 9 2 76.29 1 9 A PHE 10 2 74.24 1 10 A MET 11 2 74.97 1 11 A CYS 12 2 76.77 1 12 A LEU 13 2 73.68 1 13 A VAL 14 2 75.37 1 14 A LYS 15 2 67.54 1 15 A SER 16 2 70.23 1 16 # _ma_software_group.group_id 1 _ma_software_group.ordinal_id 1 _ma_software_group.software_id 1 # _ma_target_entity.data_id 1 _ma_target_entity.entity_id 1 _ma_target_entity.origin . # _ma_target_entity_instance.asym_id A _ma_target_entity_instance.details . _ma_target_entity_instance.entity_id 1 # loop_ _pdbx_data_usage.details _pdbx_data_usage.id _pdbx_data_usage.type _pdbx_data_usage.url ;Non-commercial use only, by using this file you agree to the terms of use found at https://github.com/google-deepmind/alphafold3/blob/main/OUTPUT_TERMS_OF_USE.md. To request access to the AlphaFold 3 model parameters, follow the process set out at https://github.com/google-deepmind/alphafold3. You may only use these if received directly from Google. Use is subject to terms of use available at https://github.com/google-deepmind/alphafold3/blob/main/WEIGHTS_TERMS_OF_USE.md. ; 1 license https://github.com/google-deepmind/alphafold3/blob/main/OUTPUT_TERMS_OF_USE.md ;AlphaFold 3 and its output are not intended for, have not been validated for, and are not approved for clinical use. They are provided "as-is" without any warranty of any kind, whether expressed or implied. No warranty is given that use shall not infringe the rights of any third party. ; 2 disclaimer ? # loop_ _pdbx_poly_seq_scheme.asym_id _pdbx_poly_seq_scheme.auth_seq_num _pdbx_poly_seq_scheme.entity_id _pdbx_poly_seq_scheme.hetero _pdbx_poly_seq_scheme.mon_id _pdbx_poly_seq_scheme.pdb_ins_code _pdbx_poly_seq_scheme.pdb_seq_num _pdbx_poly_seq_scheme.pdb_strand_id _pdbx_poly_seq_scheme.seq_id A 1 1 n MET . 1 A 1 A 2 1 n LEU . 2 A 2 A 3 1 n ARG . 3 A 3 A 4 1 n LEU . 4 A 4 A 5 1 n CYS . 5 A 5 A 6 1 n PHE . 6 A 6 A 7 1 n LEU . 7 A 7 A 8 1 n LEU . 8 A 8 A 9 1 n SER . 9 A 9 A 10 1 n PHE . 10 A 10 A 11 1 n MET . 11 A 11 A 12 1 n CYS . 12 A 12 A 13 1 n LEU . 13 A 13 A 14 1 n VAL . 14 A 14 A 15 1 n LYS . 15 A 15 A 16 1 n SER . 16 A 16 # _software.classification other _software.date ? _software.description "Structure prediction" _software.name AlphaFold _software.pdbx_ordinal 1 _software.type package _software.version "AlphaFold-beta-20231127 (9ceee3b5f7825b611ab42eaad843248507ced2da1e745fa3b3d93c6c4ad3ca74)" # _struct_asym.entity_id 1 _struct_asym.id A # loop_ _atom_site.group_PDB _atom_site.id _atom_site.type_symbol _atom_site.label_atom_id _atom_site.label_alt_id _atom_site.label_comp_id _atom_site.label_asym_id _atom_site.label_entity_id _atom_site.label_seq_id _atom_site.pdbx_PDB_ins_code _atom_site.Cartn_x _atom_site.Cartn_y _atom_site.Cartn_z _atom_site.occupancy _atom_site.B_iso_or_equiv _atom_site.auth_seq_id _atom_site.auth_asym_id _atom_site.pdbx_PDB_model_num ATOM 1 N N . MET A 1 1 ? -11.914 -4.303 -0.135 1.00 69.61 1 A 1 ATOM 2 C CA . MET A 1 1 ? -11.675 -3.448 1.044 1.00 73.21 1 A 1 ATOM 3 C C . MET A 1 1 ? -10.258 -3.640 1.562 1.00 73.57 1 A 1 ATOM 4 O O . MET A 1 1 ? -9.494 -2.680 1.669 1.00 71.62 1 A 1 ATOM 5 C CB . MET A 1 1 ? -12.688 -3.759 2.154 1.00 68.18 1 A 1 ATOM 6 C CG . MET A 1 1 ? -12.677 -2.711 3.261 1.00 63.11 1 A 1 ATOM 7 S SD . MET A 1 1 ? -13.949 -3.001 4.491 1.00 58.11 1 A 1 ATOM 8 C CE . MET A 1 1 ? -13.806 -1.500 5.465 1.00 52.30 1 A 1 ATOM 9 N N . LEU A 1 2 ? -9.914 -4.872 1.876 1.00 69.06 2 A 1 ATOM 10 C CA . LEU A 1 2 ? -8.568 -5.169 2.355 1.00 72.51 2 A 1 ATOM 11 C C . LEU A 1 2 ? -7.535 -4.845 1.293 1.00 75.34 2 A 1 ATOM 12 O O . LEU A 1 2 ? -6.464 -4.314 1.597 1.00 72.37 2 A 1 ATOM 13 C CB . LEU A 1 2 ? -8.459 -6.644 2.757 1.00 70.30 2 A 1 ATOM 14 C CG . LEU A 1 2 ? -7.086 -7.037 3.307 1.00 65.82 2 A 1 ATOM 15 C CD1 . LEU A 1 2 ? -6.773 -6.279 4.593 1.00 63.81 2 A 1 ATOM 16 C CD2 . LEU A 1 2 ? -7.039 -8.533 3.552 1.00 64.48 2 A 1 ATOM 17 N N . ARG A 1 3 ? -7.880 -5.154 0.060 1.00 72.13 3 A 1 ATOM 18 C CA . ARG A 1 3 ? -6.977 -4.875 -1.052 1.00 75.60 3 A 1 ATOM 19 C C . ARG A 1 3 ? -6.742 -3.381 -1.192 1.00 76.11 3 A 1 ATOM 20 O O . ARG A 1 3 ? -5.614 -2.937 -1.387 1.00 74.57 3 A 1 ATOM 21 C CB . ARG A 1 3 ? -7.550 -5.451 -2.348 1.00 75.09 3 A 1 ATOM 22 C CG . ARG A 1 3 ? -7.426 -6.960 -2.399 1.00 69.44 3 A 1 ATOM 23 C CD . ARG A 1 3 ? -8.302 -7.549 -3.473 1.00 68.19 3 A 1 ATOM 24 N NE . ARG A 1 3 ? -9.718 -7.467 -3.093 1.00 64.77 3 A 1 ATOM 25 C CZ . ARG A 1 3 ? -10.690 -8.078 -3.756 1.00 58.21 3 A 1 ATOM 26 N NH1 . ARG A 1 3 ? -10.436 -8.798 -4.829 1.00 54.86 3 A 1 ATOM 27 N NH2 . ARG A 1 3 ? -11.940 -7.954 -3.342 1.00 54.79 3 A 1 ATOM 28 N N . LEU A 1 4 ? -7.797 -2.605 -1.065 1.00 79.25 4 A 1 ATOM 29 C CA . LEU A 1 4 ? -7.688 -1.154 -1.149 1.00 81.51 4 A 1 ATOM 30 C C . LEU A 1 4 ? -6.890 -0.611 0.025 1.00 83.15 4 A 1 ATOM 31 O O . LEU A 1 4 ? -6.000 0.219 -0.151 1.00 81.40 4 A 1 ATOM 32 C CB . LEU A 1 4 ? -9.079 -0.514 -1.164 1.00 81.23 4 A 1 ATOM 33 C CG . LEU A 1 4 ? -9.899 -0.836 -2.402 1.00 76.42 4 A 1 ATOM 34 C CD1 . LEU A 1 4 ? -11.316 -0.310 -2.229 1.00 71.41 4 A 1 ATOM 35 C CD2 . LEU A 1 4 ? -9.262 -0.217 -3.641 1.00 71.84 4 A 1 ATOM 36 N N . CYS A 1 5 ? -7.197 -1.088 1.220 1.00 80.97 5 A 1 ATOM 37 C CA . CYS A 1 5 ? -6.491 -0.668 2.423 1.00 81.87 5 A 1 ATOM 38 C C . CYS A 1 5 ? -5.026 -1.073 2.344 1.00 83.14 5 A 1 ATOM 39 O O . CYS A 1 5 ? -4.140 -0.313 2.728 1.00 79.94 5 A 1 ATOM 40 C CB . CYS A 1 5 ? -7.131 -1.286 3.666 1.00 78.60 5 A 1 ATOM 41 S SG . CYS A 1 5 ? -6.370 -0.698 5.192 1.00 70.37 5 A 1 ATOM 42 N N . PHE A 1 6 ? -4.781 -2.270 1.832 1.00 76.62 6 A 1 ATOM 43 C CA . PHE A 1 6 ? -3.425 -2.775 1.672 1.00 78.55 6 A 1 ATOM 44 C C . PHE A 1 6 ? -2.650 -1.913 0.689 1.00 80.26 6 A 1 ATOM 45 O O . PHE A 1 6 ? -1.497 -1.563 0.940 1.00 78.75 6 A 1 ATOM 46 C CB . PHE A 1 6 ? -3.460 -4.223 1.186 1.00 78.31 6 A 1 ATOM 47 C CG . PHE A 1 6 ? -2.088 -4.823 1.034 1.00 73.91 6 A 1 ATOM 48 C CD1 . PHE A 1 6 ? -1.340 -5.168 2.147 1.00 70.77 6 A 1 ATOM 49 C CD2 . PHE A 1 6 ? -1.554 -5.027 -0.226 1.00 71.54 6 A 1 ATOM 50 C CE1 . PHE A 1 6 ? -0.073 -5.714 2.009 1.00 66.71 6 A 1 ATOM 51 C CE2 . PHE A 1 6 ? -0.281 -5.575 -0.371 1.00 67.60 6 A 1 ATOM 52 C CZ . PHE A 1 6 ? 0.454 -5.917 0.749 1.00 68.08 6 A 1 ATOM 53 N N . LEU A 1 7 ? -3.276 -1.566 -0.411 1.00 80.87 7 A 1 ATOM 54 C CA . LEU A 1 7 ? -2.643 -0.725 -1.416 1.00 81.65 7 A 1 ATOM 55 C C . LEU A 1 7 ? -2.345 0.657 -0.860 1.00 82.27 7 A 1 ATOM 56 O O . LEU A 1 7 ? -1.262 1.195 -1.077 1.00 81.83 7 A 1 ATOM 57 C CB . LEU A 1 7 ? -3.540 -0.607 -2.646 1.00 82.61 7 A 1 ATOM 58 C CG . LEU A 1 7 ? -3.644 -1.889 -3.461 1.00 77.09 7 A 1 ATOM 59 C CD1 . LEU A 1 7 ? -4.737 -1.759 -4.508 1.00 72.58 7 A 1 ATOM 60 C CD2 . LEU A 1 7 ? -2.315 -2.199 -4.137 1.00 74.64 7 A 1 ATOM 61 N N . LEU A 1 8 ? -3.294 1.219 -0.150 1.00 81.14 8 A 1 ATOM 62 C CA . LEU A 1 8 ? -3.118 2.538 0.446 1.00 81.41 8 A 1 ATOM 63 C C . LEU A 1 8 ? -1.995 2.522 1.470 1.00 82.15 8 A 1 ATOM 64 O O . LEU A 1 8 ? -1.115 3.381 1.452 1.00 80.37 8 A 1 ATOM 65 C CB . LEU A 1 8 ? -4.416 2.999 1.109 1.00 81.56 8 A 1 ATOM 66 C CG . LEU A 1 8 ? -5.533 3.320 0.130 1.00 76.64 8 A 1 ATOM 67 C CD1 . LEU A 1 8 ? -6.834 3.551 0.889 1.00 71.24 8 A 1 ATOM 68 C CD2 . LEU A 1 8 ? -5.189 4.554 -0.697 1.00 73.18 8 A 1 ATOM 69 N N . SER A 1 9 ? -2.017 1.539 2.358 1.00 77.81 9 A 1 ATOM 70 C CA . SER A 1 9 ? -0.983 1.412 3.380 1.00 78.32 9 A 1 ATOM 71 C C . SER A 1 9 ? 0.363 1.124 2.737 1.00 78.16 9 A 1 ATOM 72 O O . SER A 1 9 ? 1.379 1.704 3.113 1.00 77.27 9 A 1 ATOM 73 C CB . SER A 1 9 ? -1.334 0.300 4.361 1.00 77.50 9 A 1 ATOM 74 O OG . SER A 1 9 ? -2.517 0.618 5.061 1.00 68.67 9 A 1 ATOM 75 N N . PHE A 1 10 ? 0.360 0.237 1.763 1.00 75.94 10 A 1 ATOM 76 C CA . PHE A 1 10 ? 1.580 -0.118 1.052 1.00 77.04 10 A 1 ATOM 77 C C . PHE A 1 10 ? 2.167 1.100 0.359 1.00 78.09 10 A 1 ATOM 78 O O . PHE A 1 10 ? 3.368 1.347 0.442 1.00 76.36 10 A 1 ATOM 79 C CB . PHE A 1 10 ? 1.280 -1.211 0.031 1.00 77.75 10 A 1 ATOM 80 C CG . PHE A 1 10 ? 2.515 -1.710 -0.666 1.00 73.82 10 A 1 ATOM 81 C CD1 . PHE A 1 10 ? 3.462 -2.443 0.022 1.00 72.13 10 A 1 ATOM 82 C CD2 . PHE A 1 10 ? 2.715 -1.440 -2.009 1.00 73.77 10 A 1 ATOM 83 C CE1 . PHE A 1 10 ? 4.601 -2.904 -0.617 1.00 69.71 10 A 1 ATOM 84 C CE2 . PHE A 1 10 ? 3.855 -1.902 -2.659 1.00 70.74 10 A 1 ATOM 85 C CZ . PHE A 1 10 ? 4.795 -2.633 -1.959 1.00 71.27 10 A 1 ATOM 86 N N . MET A 1 11 ? 1.320 1.866 -0.312 1.00 78.05 11 A 1 ATOM 87 C CA . MET A 1 11 ? 1.763 3.082 -0.988 1.00 78.08 11 A 1 ATOM 88 C C . MET A 1 11 ? 2.328 4.074 0.018 1.00 78.65 11 A 1 ATOM 89 O O . MET A 1 11 ? 3.358 4.693 -0.222 1.00 75.55 11 A 1 ATOM 90 C CB . MET A 1 11 ? 0.593 3.723 -1.746 1.00 78.64 11 A 1 ATOM 91 C CG . MET A 1 11 ? 0.210 2.936 -2.998 1.00 76.46 11 A 1 ATOM 92 S SD . MET A 1 11 ? 1.434 3.125 -4.293 1.00 68.98 11 A 1 ATOM 93 C CE . MET A 1 11 ? 0.679 2.165 -5.600 1.00 65.37 11 A 1 ATOM 94 N N . CYS A 1 12 ? 1.665 4.222 1.137 1.00 79.49 12 A 1 ATOM 95 C CA . CYS A 1 12 ? 2.108 5.140 2.181 1.00 78.94 12 A 1 ATOM 96 C C . CYS A 1 12 ? 3.435 4.689 2.778 1.00 79.79 12 A 1 ATOM 97 O O . CYS A 1 12 ? 4.264 5.515 3.153 1.00 75.33 12 A 1 ATOM 98 C CB . CYS A 1 12 ? 1.053 5.240 3.284 1.00 77.00 12 A 1 ATOM 99 S SG . CYS A 1 12 ? 1.458 6.505 4.498 1.00 70.07 12 A 1 ATOM 100 N N . LEU A 1 13 ? 3.636 3.379 2.851 1.00 77.02 13 A 1 ATOM 101 C CA . LEU A 1 13 ? 4.865 2.834 3.414 1.00 77.92 13 A 1 ATOM 102 C C . LEU A 1 13 ? 6.021 2.908 2.425 1.00 78.64 13 A 1 ATOM 103 O O . LEU A 1 13 ? 7.055 3.506 2.721 1.00 76.00 13 A 1 ATOM 104 C CB . LEU A 1 13 ? 4.638 1.382 3.845 1.00 77.37 13 A 1 ATOM 105 C CG . LEU A 1 13 ? 3.717 1.230 5.046 1.00 69.43 13 A 1 ATOM 106 C CD1 . LEU A 1 13 ? 3.367 -0.232 5.266 1.00 65.97 13 A 1 ATOM 107 C CD2 . LEU A 1 13 ? 4.381 1.797 6.290 1.00 67.06 13 A 1 ATOM 108 N N . VAL A 1 14 ? 5.858 2.296 1.278 1.00 78.86 14 A 1 ATOM 109 C CA . VAL A 1 14 ? 6.930 2.265 0.282 1.00 78.97 14 A 1 ATOM 110 C C . VAL A 1 14 ? 7.144 3.620 -0.378 1.00 78.67 14 A 1 ATOM 111 O O . VAL A 1 14 ? 8.277 4.005 -0.667 1.00 75.57 14 A 1 ATOM 112 C CB . VAL A 1 14 ? 6.662 1.196 -0.793 1.00 76.65 14 A 1 ATOM 113 C CG1 . VAL A 1 14 ? 6.550 -0.169 -0.135 1.00 68.85 14 A 1 ATOM 114 C CG2 . VAL A 1 14 ? 5.419 1.505 -1.598 1.00 70.04 14 A 1 ATOM 115 N N . LYS A 1 15 ? 6.051 4.345 -0.604 1.00 77.20 15 A 1 ATOM 116 C CA . LYS A 1 15 ? 6.133 5.663 -1.233 1.00 74.20 15 A 1 ATOM 117 C C . LYS A 1 15 ? 6.230 6.761 -0.194 1.00 71.89 15 A 1 ATOM 118 O O . LYS A 1 15 ? 6.301 7.936 -0.543 1.00 67.25 15 A 1 ATOM 119 C CB . LYS A 1 15 ? 4.912 5.895 -2.140 1.00 72.51 15 A 1 ATOM 120 C CG . LYS A 1 15 ? 4.770 4.868 -3.249 1.00 66.72 15 A 1 ATOM 121 C CD . LYS A 1 15 ? 5.813 5.064 -4.342 1.00 63.82 15 A 1 ATOM 122 C CE . LYS A 1 15 ? 5.608 6.373 -5.089 1.00 60.89 15 A 1 ATOM 123 N NZ . LYS A 1 15 ? 6.540 6.516 -6.224 1.00 53.42 15 A 1 ATOM 124 N N . SER A 1 16 ? 6.254 6.350 1.083 1.00 78.66 16 A 1 ATOM 125 C CA . SER A 1 16 ? 6.312 7.305 2.192 1.00 75.85 16 A 1 ATOM 126 C C . SER A 1 16 ? 5.160 8.299 2.105 1.00 70.84 16 A 1 ATOM 127 O O . SER A 1 16 ? 4.052 7.948 2.558 1.00 65.92 16 A 1 ATOM 128 C CB . SER A 1 16 ? 7.655 8.049 2.199 1.00 70.51 16 A 1 ATOM 129 O OG . SER A 1 16 ? 8.720 7.160 2.343 1.00 64.62 16 A 1 ATOM 130 O OXT . SER A 1 16 ? 5.319 9.393 1.584 1.00 65.18 16 A 1 #