# By using this file you agree to the legally binding terms of use found at # https://github.com/google-deepmind/alphafold3/blob/main/OUTPUT_TERMS_OF_USE.md. # To request access to the AlphaFold 3 model parameters, follow the process set # out at https://github.com/google-deepmind/alphafold3. You may only use these if # received directly from Google. Use is subject to terms of use available at # https://github.com/google-deepmind/alphafold3/blob/main/WEIGHTS_TERMS_OF_USE.md. data_spkb28287 # _entry.id spkb28287 # loop_ _atom_type.symbol C N O S # loop_ _audit_author.name _audit_author.pdbx_ordinal "Google DeepMind" 1 "Isomorphic Labs" 2 # _audit_conform.dict_location https://raw.githubusercontent.com/ihmwg/ModelCIF/master/dist/mmcif_ma.dic _audit_conform.dict_name mmcif_ma.dic _audit_conform.dict_version 1.4.5 # loop_ _chem_comp.formula _chem_comp.formula_weight _chem_comp.id _chem_comp.mon_nstd_flag _chem_comp.name _chem_comp.pdbx_smiles _chem_comp.pdbx_synonyms _chem_comp.type "C3 H7 N O2" 89.093 ALA y ALANINE C[C@@H](C(=O)O)N ? "L-PEPTIDE LINKING" "C6 H10 N3 O2" 156.162 HIS y HISTIDINE c1c([nH+]c[nH]1)C[C@@H](C(=O)O)N ? "L-PEPTIDE LINKING" "C6 H13 N O2" 131.173 LEU y LEUCINE CC(C)C[C@@H](C(=O)O)N ? "L-PEPTIDE LINKING" "C5 H11 N O2 S" 149.211 MET y METHIONINE CSCC[C@@H](C(=O)O)N ? "L-PEPTIDE LINKING" "C9 H11 N O2" 165.189 PHE y PHENYLALANINE c1ccc(cc1)C[C@@H](C(=O)O)N ? "L-PEPTIDE LINKING" "C3 H7 N O3" 105.093 SER y SERINE C([C@@H](C(=O)O)N)O ? "L-PEPTIDE LINKING" "C9 H11 N O3" 181.189 TYR y TYROSINE c1cc(ccc1C[C@@H](C(=O)O)N)O ? "L-PEPTIDE LINKING" "C5 H11 N O2" 117.146 VAL y VALINE CC(C)[C@@H](C(=O)O)N ? "L-PEPTIDE LINKING" # _citation.book_publisher ? _citation.country UK _citation.id primary _citation.journal_full Nature _citation.journal_id_ASTM NATUAS _citation.journal_id_CSD 0006 _citation.journal_id_ISSN 0028-0836 _citation.journal_volume 630 _citation.page_first 493 _citation.page_last 500 _citation.pdbx_database_id_DOI 10.1038/s41586-024-07487-w _citation.pdbx_database_id_PubMed 38718835 _citation.title "Accurate structure prediction of biomolecular interactions with AlphaFold 3" _citation.year 2024 # loop_ _citation_author.citation_id _citation_author.name _citation_author.ordinal primary "Google DeepMind" 1 primary "Isomorphic Labs" 2 # _entity.id 1 _entity.pdbx_description . _entity.type polymer # _entity_poly.entity_id 1 _entity_poly.pdbx_strand_id A _entity_poly.type polypeptide(L) # loop_ _entity_poly_seq.entity_id _entity_poly_seq.hetero _entity_poly_seq.mon_id _entity_poly_seq.num 1 n MET 1 1 n HIS 2 1 n LEU 3 1 n LEU 4 1 n VAL 5 1 n TYR 6 1 n LEU 7 1 n SER 8 1 n LEU 9 1 n PHE 10 1 n PHE 11 1 n ALA 12 1 n LEU 13 1 n ALA 14 1 n LEU 15 1 n ALA 16 # _ma_data.content_type "model coordinates" _ma_data.id 1 _ma_data.name Model # _ma_model_list.data_id 1 _ma_model_list.model_group_id 1 _ma_model_list.model_group_name "AlphaFold-beta-20231127 (3.0.0 @ 2025-07-11 21:45:41)" _ma_model_list.model_id 1 _ma_model_list.model_name "Top ranked model" _ma_model_list.model_type "Ab initio model" _ma_model_list.ordinal_id 1 # loop_ _ma_protocol_step.method_type _ma_protocol_step.ordinal_id _ma_protocol_step.protocol_id _ma_protocol_step.step_id "coevolution MSA" 1 1 1 "template search" 2 1 2 modeling 3 1 3 # loop_ _ma_qa_metric.id _ma_qa_metric.mode _ma_qa_metric.name _ma_qa_metric.software_group_id _ma_qa_metric.type 1 global pLDDT 1 pLDDT 2 local pLDDT 1 pLDDT # _ma_qa_metric_global.metric_id 1 _ma_qa_metric_global.metric_value 93.66 _ma_qa_metric_global.model_id 1 _ma_qa_metric_global.ordinal_id 1 # loop_ _ma_qa_metric_local.label_asym_id _ma_qa_metric_local.label_comp_id _ma_qa_metric_local.label_seq_id _ma_qa_metric_local.metric_id _ma_qa_metric_local.metric_value _ma_qa_metric_local.model_id _ma_qa_metric_local.ordinal_id A MET 1 2 81.91 1 1 A HIS 2 2 86.99 1 2 A LEU 3 2 94.88 1 3 A LEU 4 2 95.45 1 4 A VAL 5 2 97.10 1 5 A TYR 6 2 94.94 1 6 A LEU 7 2 95.95 1 7 A SER 8 2 96.36 1 8 A LEU 9 2 96.13 1 9 A PHE 10 2 94.68 1 10 A PHE 11 2 93.21 1 11 A ALA 12 2 98.15 1 12 A LEU 13 2 95.57 1 13 A ALA 14 2 97.48 1 14 A LEU 15 2 93.30 1 15 A ALA 16 2 90.84 1 16 # _ma_software_group.group_id 1 _ma_software_group.ordinal_id 1 _ma_software_group.software_id 1 # _ma_target_entity.data_id 1 _ma_target_entity.entity_id 1 _ma_target_entity.origin . # _ma_target_entity_instance.asym_id A _ma_target_entity_instance.details . _ma_target_entity_instance.entity_id 1 # loop_ _pdbx_data_usage.details _pdbx_data_usage.id _pdbx_data_usage.type _pdbx_data_usage.url ;Non-commercial use only, by using this file you agree to the terms of use found at https://github.com/google-deepmind/alphafold3/blob/main/OUTPUT_TERMS_OF_USE.md. To request access to the AlphaFold 3 model parameters, follow the process set out at https://github.com/google-deepmind/alphafold3. You may only use these if received directly from Google. Use is subject to terms of use available at https://github.com/google-deepmind/alphafold3/blob/main/WEIGHTS_TERMS_OF_USE.md. ; 1 license https://github.com/google-deepmind/alphafold3/blob/main/OUTPUT_TERMS_OF_USE.md ;AlphaFold 3 and its output are not intended for, have not been validated for, and are not approved for clinical use. They are provided "as-is" without any warranty of any kind, whether expressed or implied. No warranty is given that use shall not infringe the rights of any third party. ; 2 disclaimer ? # loop_ _pdbx_poly_seq_scheme.asym_id _pdbx_poly_seq_scheme.auth_seq_num _pdbx_poly_seq_scheme.entity_id _pdbx_poly_seq_scheme.hetero _pdbx_poly_seq_scheme.mon_id _pdbx_poly_seq_scheme.pdb_ins_code _pdbx_poly_seq_scheme.pdb_seq_num _pdbx_poly_seq_scheme.pdb_strand_id _pdbx_poly_seq_scheme.seq_id A 1 1 n MET . 1 A 1 A 2 1 n HIS . 2 A 2 A 3 1 n LEU . 3 A 3 A 4 1 n LEU . 4 A 4 A 5 1 n VAL . 5 A 5 A 6 1 n TYR . 6 A 6 A 7 1 n LEU . 7 A 7 A 8 1 n SER . 8 A 8 A 9 1 n LEU . 9 A 9 A 10 1 n PHE . 10 A 10 A 11 1 n PHE . 11 A 11 A 12 1 n ALA . 12 A 12 A 13 1 n LEU . 13 A 13 A 14 1 n ALA . 14 A 14 A 15 1 n LEU . 15 A 15 A 16 1 n ALA . 16 A 16 # _software.classification other _software.date ? _software.description "Structure prediction" _software.name AlphaFold _software.pdbx_ordinal 1 _software.type package _software.version "AlphaFold-beta-20231127 (9ceee3b5f7825b611ab42eaad843248507ced2da1e745fa3b3d93c6c4ad3ca74)" # _struct_asym.entity_id 1 _struct_asym.id A # loop_ _atom_site.group_PDB _atom_site.id _atom_site.type_symbol _atom_site.label_atom_id _atom_site.label_alt_id _atom_site.label_comp_id _atom_site.label_asym_id _atom_site.label_entity_id _atom_site.label_seq_id _atom_site.pdbx_PDB_ins_code _atom_site.Cartn_x _atom_site.Cartn_y _atom_site.Cartn_z _atom_site.occupancy _atom_site.B_iso_or_equiv _atom_site.auth_seq_id _atom_site.auth_asym_id _atom_site.pdbx_PDB_model_num ATOM 1 N N . MET A 1 1 ? -9.966 -2.543 7.657 1.00 87.69 1 A 1 ATOM 2 C CA . MET A 1 1 ? -9.646 -2.334 6.232 1.00 88.90 1 A 1 ATOM 3 C C . MET A 1 1 ? -8.147 -2.494 5.980 1.00 89.53 1 A 1 ATOM 4 O O . MET A 1 1 ? -7.375 -1.563 6.180 1.00 86.71 1 A 1 ATOM 5 C CB . MET A 1 1 ? -10.081 -0.937 5.802 1.00 83.87 1 A 1 ATOM 6 C CG . MET A 1 1 ? -11.548 -0.870 5.437 1.00 78.16 1 A 1 ATOM 7 S SD . MET A 1 1 ? -12.132 0.822 5.316 1.00 74.75 1 A 1 ATOM 8 C CE . MET A 1 1 ? -13.894 0.571 5.380 1.00 65.65 1 A 1 ATOM 9 N N . HIS A 1 2 ? -7.763 -3.688 5.563 1.00 91.72 2 A 1 ATOM 10 C CA . HIS A 1 2 ? -6.363 -3.966 5.272 1.00 94.48 2 A 1 ATOM 11 C C . HIS A 1 2 ? -6.044 -3.722 3.799 1.00 96.31 2 A 1 ATOM 12 O O . HIS A 1 2 ? -4.895 -3.477 3.438 1.00 95.27 2 A 1 ATOM 13 C CB . HIS A 1 2 ? -6.033 -5.412 5.643 1.00 92.01 2 A 1 ATOM 14 C CG . HIS A 1 2 ? -5.182 -5.505 6.869 1.00 87.39 2 A 1 ATOM 15 N ND1 . HIS A 1 2 ? -5.398 -6.420 7.867 1.00 79.53 2 A 1 ATOM 16 C CD2 . HIS A 1 2 ? -4.099 -4.784 7.246 1.00 75.39 2 A 1 ATOM 17 C CE1 . HIS A 1 2 ? -4.488 -6.259 8.805 1.00 78.86 2 A 1 ATOM 18 N NE2 . HIS A 1 2 ? -3.682 -5.268 8.452 1.00 78.96 2 A 1 ATOM 19 N N . LEU A 1 3 ? -7.070 -3.796 2.954 1.00 96.46 3 A 1 ATOM 20 C CA . LEU A 1 3 ? -6.887 -3.563 1.522 1.00 97.06 3 A 1 ATOM 21 C C . LEU A 1 3 ? -6.428 -2.137 1.261 1.00 97.79 3 A 1 ATOM 22 O O . LEU A 1 3 ? -5.574 -1.893 0.406 1.00 97.31 3 A 1 ATOM 23 C CB . LEU A 1 3 ? -8.194 -3.837 0.777 1.00 96.74 3 A 1 ATOM 24 C CG . LEU A 1 3 ? -8.439 -5.308 0.479 1.00 93.28 3 A 1 ATOM 25 C CD1 . LEU A 1 3 ? -9.894 -5.531 0.105 1.00 90.36 3 A 1 ATOM 26 C CD2 . LEU A 1 3 ? -7.530 -5.772 -0.654 1.00 90.01 3 A 1 ATOM 27 N N . LEU A 1 4 ? -6.997 -1.201 1.999 1.00 97.01 4 A 1 ATOM 28 C CA . LEU A 1 4 ? -6.641 0.210 1.857 1.00 97.55 4 A 1 ATOM 29 C C . LEU A 1 4 ? -5.179 0.422 2.237 1.00 97.88 4 A 1 ATOM 30 O O . LEU A 1 4 ? -4.455 1.173 1.585 1.00 97.61 4 A 1 ATOM 31 C CB . LEU A 1 4 ? -7.560 1.073 2.731 1.00 97.41 4 A 1 ATOM 32 C CG . LEU A 1 4 ? -7.923 2.432 2.127 1.00 94.00 4 A 1 ATOM 33 C CD1 . LEU A 1 4 ? -6.700 3.311 1.949 1.00 91.13 4 A 1 ATOM 34 C CD2 . LEU A 1 4 ? -8.643 2.235 0.801 1.00 91.04 4 A 1 ATOM 35 N N . VAL A 1 5 ? -4.759 -0.243 3.289 1.00 97.80 5 A 1 ATOM 36 C CA . VAL A 1 5 ? -3.375 -0.145 3.758 1.00 97.90 5 A 1 ATOM 37 C C . VAL A 1 5 ? -2.416 -0.722 2.718 1.00 98.10 5 A 1 ATOM 38 O O . VAL A 1 5 ? -1.337 -0.175 2.482 1.00 97.93 5 A 1 ATOM 39 C CB . VAL A 1 5 ? -3.200 -0.880 5.096 1.00 97.52 5 A 1 ATOM 40 C CG1 . VAL A 1 5 ? -1.771 -0.734 5.595 1.00 95.32 5 A 1 ATOM 41 C CG2 . VAL A 1 5 ? -4.173 -0.340 6.128 1.00 95.12 5 A 1 ATOM 42 N N . TYR A 1 6 ? -2.824 -1.826 2.114 1.00 97.97 6 A 1 ATOM 43 C CA . TYR A 1 6 ? -2.006 -2.467 1.088 1.00 98.05 6 A 1 ATOM 44 C C . TYR A 1 6 ? -1.804 -1.541 -0.103 1.00 98.18 6 A 1 ATOM 45 O O . TYR A 1 6 ? -0.689 -1.386 -0.608 1.00 98.06 6 A 1 ATOM 46 C CB . TYR A 1 6 ? -2.682 -3.757 0.622 1.00 97.84 6 A 1 ATOM 47 C CG . TYR A 1 6 ? -1.920 -4.989 1.036 1.00 95.22 6 A 1 ATOM 48 C CD1 . TYR A 1 6 ? -1.864 -5.375 2.367 1.00 92.89 6 A 1 ATOM 49 C CD2 . TYR A 1 6 ? -1.260 -5.761 0.090 1.00 92.77 6 A 1 ATOM 50 C CE1 . TYR A 1 6 ? -1.162 -6.504 2.750 1.00 92.19 6 A 1 ATOM 51 C CE2 . TYR A 1 6 ? -0.557 -6.894 0.464 1.00 92.37 6 A 1 ATOM 52 C CZ . TYR A 1 6 ? -0.513 -7.261 1.795 1.00 92.47 6 A 1 ATOM 53 O OH . TYR A 1 6 ? 0.183 -8.384 2.168 1.00 91.24 6 A 1 ATOM 54 N N . LEU A 1 7 ? -2.887 -0.929 -0.539 1.00 97.84 7 A 1 ATOM 55 C CA . LEU A 1 7 ? -2.828 -0.003 -1.668 1.00 97.90 7 A 1 ATOM 56 C C . LEU A 1 7 ? -1.968 1.209 -1.332 1.00 98.10 7 A 1 ATOM 57 O O . LEU A 1 7 ? -1.195 1.682 -2.166 1.00 98.03 7 A 1 ATOM 58 C CB . LEU A 1 7 ? -4.241 0.447 -2.041 1.00 97.82 7 A 1 ATOM 59 C CG . LEU A 1 7 ? -4.921 -0.460 -3.059 1.00 94.19 7 A 1 ATOM 60 C CD1 . LEU A 1 7 ? -6.430 -0.292 -2.993 1.00 91.38 7 A 1 ATOM 61 C CD2 . LEU A 1 7 ? -4.417 -0.141 -4.460 1.00 92.37 7 A 1 ATOM 62 N N . SER A 1 8 ? -2.116 1.705 -0.122 1.00 97.74 8 A 1 ATOM 63 C CA . SER A 1 8 ? -1.354 2.863 0.328 1.00 97.82 8 A 1 ATOM 64 C C . SER A 1 8 ? 0.142 2.564 0.321 1.00 97.99 8 A 1 ATOM 65 O O . SER A 1 8 ? 0.949 3.388 -0.107 1.00 97.61 8 A 1 ATOM 66 C CB . SER A 1 8 ? -1.802 3.275 1.727 1.00 97.50 8 A 1 ATOM 67 O OG . SER A 1 8 ? -1.228 4.511 2.078 1.00 89.49 8 A 1 ATOM 68 N N . LEU A 1 9 ? 0.495 1.380 0.788 1.00 97.83 9 A 1 ATOM 69 C CA . LEU A 1 9 ? 1.895 0.967 0.830 1.00 97.75 9 A 1 ATOM 70 C C . LEU A 1 9 ? 2.460 0.827 -0.578 1.00 97.93 9 A 1 ATOM 71 O O . LEU A 1 9 ? 3.558 1.302 -0.868 1.00 97.75 9 A 1 ATOM 72 C CB . LEU A 1 9 ? 2.024 -0.367 1.571 1.00 97.60 9 A 1 ATOM 73 C CG . LEU A 1 9 ? 3.368 -0.548 2.269 1.00 94.38 9 A 1 ATOM 74 C CD1 . LEU A 1 9 ? 3.220 -0.338 3.765 1.00 92.51 9 A 1 ATOM 75 C CD2 . LEU A 1 9 ? 3.938 -1.926 1.985 1.00 93.28 9 A 1 ATOM 76 N N . PHE A 1 10 ? 1.698 0.165 -1.439 1.00 97.50 10 A 1 ATOM 77 C CA . PHE A 1 10 ? 2.104 -0.028 -2.827 1.00 97.41 10 A 1 ATOM 78 C C . PHE A 1 10 ? 2.250 1.307 -3.545 1.00 97.67 10 A 1 ATOM 79 O O . PHE A 1 10 ? 3.193 1.515 -4.312 1.00 97.41 10 A 1 ATOM 80 C CB . PHE A 1 10 ? 1.073 -0.897 -3.542 1.00 96.96 10 A 1 ATOM 81 C CG . PHE A 1 10 ? 1.261 -0.907 -5.035 1.00 95.08 10 A 1 ATOM 82 C CD1 . PHE A 1 10 ? 2.327 -1.580 -5.602 1.00 92.51 10 A 1 ATOM 83 C CD2 . PHE A 1 10 ? 0.374 -0.235 -5.856 1.00 92.79 10 A 1 ATOM 84 C CE1 . PHE A 1 10 ? 2.510 -1.586 -6.977 1.00 91.29 10 A 1 ATOM 85 C CE2 . PHE A 1 10 ? 0.555 -0.234 -7.234 1.00 91.24 10 A 1 ATOM 86 C CZ . PHE A 1 10 ? 1.623 -0.911 -7.792 1.00 91.61 10 A 1 ATOM 87 N N . PHE A 1 11 ? 1.309 2.199 -3.298 1.00 97.14 11 A 1 ATOM 88 C CA . PHE A 1 11 ? 1.323 3.515 -3.923 1.00 96.93 11 A 1 ATOM 89 C C . PHE A 1 11 ? 2.549 4.304 -3.491 1.00 97.46 11 A 1 ATOM 90 O O . PHE A 1 11 ? 3.188 4.973 -4.302 1.00 97.21 11 A 1 ATOM 91 C CB . PHE A 1 11 ? 0.052 4.276 -3.555 1.00 96.53 11 A 1 ATOM 92 C CG . PHE A 1 11 ? -0.398 5.201 -4.650 1.00 92.91 11 A 1 ATOM 93 C CD1 . PHE A 1 11 ? -0.827 4.699 -5.865 1.00 89.92 11 A 1 ATOM 94 C CD2 . PHE A 1 11 ? -0.394 6.572 -4.450 1.00 90.60 11 A 1 ATOM 95 C CE1 . PHE A 1 11 ? -1.242 5.549 -6.876 1.00 89.32 11 A 1 ATOM 96 C CE2 . PHE A 1 11 ? -0.808 7.428 -5.457 1.00 88.63 11 A 1 ATOM 97 C CZ . PHE A 1 11 ? -1.232 6.918 -6.672 1.00 88.65 11 A 1 ATOM 98 N N . ALA A 1 12 ? 2.867 4.216 -2.211 1.00 98.18 12 A 1 ATOM 99 C CA . ALA A 1 12 ? 4.027 4.919 -1.673 1.00 98.25 12 A 1 ATOM 100 C C . ALA A 1 12 ? 5.316 4.430 -2.329 1.00 98.42 12 A 1 ATOM 101 O O . ALA A 1 12 ? 6.204 5.225 -2.636 1.00 97.94 12 A 1 ATOM 102 C CB . ALA A 1 12 ? 4.096 4.720 -0.167 1.00 97.96 12 A 1 ATOM 103 N N . LEU A 1 13 ? 5.405 3.130 -2.536 1.00 97.73 13 A 1 ATOM 104 C CA . LEU A 1 13 ? 6.575 2.537 -3.175 1.00 97.50 13 A 1 ATOM 105 C C . LEU A 1 13 ? 6.658 2.941 -4.638 1.00 97.67 13 A 1 ATOM 106 O O . LEU A 1 13 ? 7.744 3.213 -5.154 1.00 96.98 13 A 1 ATOM 107 C CB . LEU A 1 13 ? 6.515 1.012 -3.055 1.00 97.07 13 A 1 ATOM 108 C CG . LEU A 1 13 ? 7.707 0.287 -3.668 1.00 94.23 13 A 1 ATOM 109 C CD1 . LEU A 1 13 ? 8.977 0.609 -2.900 1.00 91.79 13 A 1 ATOM 110 C CD2 . LEU A 1 13 ? 7.463 -1.207 -3.684 1.00 91.60 13 A 1 ATOM 111 N N . ALA A 1 14 ? 5.519 2.966 -5.305 1.00 97.72 14 A 1 ATOM 112 C CA . ALA A 1 14 ? 5.458 3.336 -6.714 1.00 97.70 14 A 1 ATOM 113 C C . ALA A 1 14 ? 5.834 4.799 -6.916 1.00 97.84 14 A 1 ATOM 114 O O . ALA A 1 14 ? 6.482 5.148 -7.902 1.00 96.98 14 A 1 ATOM 115 C CB . ALA A 1 14 ? 4.061 3.074 -7.264 1.00 97.16 14 A 1 ATOM 116 N N . LEU A 1 15 ? 5.423 5.639 -5.982 1.00 96.21 15 A 1 ATOM 117 C CA . LEU A 1 15 ? 5.725 7.063 -6.055 1.00 96.11 15 A 1 ATOM 118 C C . LEU A 1 15 ? 7.209 7.320 -5.856 1.00 96.25 15 A 1 ATOM 119 O O . LEU A 1 15 ? 7.792 8.187 -6.505 1.00 94.52 15 A 1 ATOM 120 C CB . LEU A 1 15 ? 4.919 7.823 -5.005 1.00 94.93 15 A 1 ATOM 121 C CG . LEU A 1 15 ? 3.445 8.000 -5.350 1.00 92.14 15 A 1 ATOM 122 C CD1 . LEU A 1 15 ? 2.682 8.491 -4.136 1.00 89.11 15 A 1 ATOM 123 C CD2 . LEU A 1 15 ? 3.292 8.979 -6.509 1.00 87.16 15 A 1 ATOM 124 N N . ALA A 1 16 ? 7.805 6.571 -4.945 1.00 96.16 16 A 1 ATOM 125 C CA . ALA A 1 16 ? 9.224 6.711 -4.649 1.00 94.51 16 A 1 ATOM 126 C C . ALA A 1 16 ? 10.095 6.087 -5.741 1.00 93.28 16 A 1 ATOM 127 O O . ALA A 1 16 ? 9.575 5.344 -6.584 1.00 89.65 16 A 1 ATOM 128 C CB . ALA A 1 16 ? 9.531 6.089 -3.292 1.00 90.59 16 A 1 ATOM 129 O OXT . ALA A 1 16 ? 11.308 6.329 -5.758 1.00 80.85 16 A 1 #